Objective To establish an analytical method for the rapid determination of mold and yeast in dried rice noodles based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS).Methods GB 4789.15-2016"National Food Safety Standard Food Microbiological Inspection Mold and Yeast Enumeration"was used to detect and analyze the molds and yeasts of dried rice flour in the laboratory from 2021 to 2022,and 10 wild strains were obtained,and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS).Results According to statistics,the dried rice noodles sampled by the laboratory from 2021 to 2022 are contaminated by fungi.of which the total detection rate of mold and yeast is about 45.45％.Through MALDI-TOF MS identification,among the 10 wild strains,mold accounts for 80％and yeast accounts for 20％.Conclusion MALDI-TOF MS,as a rapid,sensitive,accurate and high-throughput microbial identification technology,has realized the rapid and specific identification of mold yeast in dried rice noodles,and can provide scientific basis for the detection of mold yeast in dried rice noodles.
dried rice noodlesmoldyeastmatrix-assisted laser desorption ionization time-of flight mass spectrometry
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