Objective Isolate and identify microorganisms that cause rancidity in vacuum packed rice dumplings,thereby helping enterprises to improve product quality and control of food safety.Methods Bromocresol purple dextrose broth and cooked meat medium base were used to cultivate the acescent rice dumplings;the microorganisms were isolated and purified by scribing in Rose bengal agar,Nutrient agar,the isolated strains were identified by colony morphology observation,cell morphology observation,16S rRNA sequence analysis,and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.The isolated strains were inoculated into commercial sterilized rice dumplings respectively to verify that two strains caused rancidity of rice dumplings.Results Two isolated strains,namely Lelliottia amnigena and Wickerhamomyces anomalus,caused rancidity of rice dumplings.Conclusion Lelliottia amnigena and Wickerhamomyces anomalus in vacuum-packed rice dumplings can cause rancidity.
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