Objective To separate and identify the miscellaneous bacteria in sterilized lactic acid bacteria beverages provided by an enterprise.Methods The miscellaneous bacteria of lactic acid bacteria beverages were isolated and purified using a variety of medias.The isolated strains were identified at the genus and species levels by analysis of 16S rRNA sequence and matrix-assisted laser desorption/ionization time of flight mass spectrometry.Results A total of 16 strains were isolated,Among them,5 strains of Acetobacter,2 strains of Gluconobacter japonicus,1 strain of Gluconacetobacter liquefaciens,1 strain of Kocuria palustris,1 strain of Streptomyces hydrogenans,1 strain of Staphylocaccus saprophyticus,1 strain of Bacillus sp,1 strain of Novosphingobium aromaticivorans,1 strain of Brevundimonas vesicularis,2 strains of Yeast,most of which(n=8,50％)were identified as Acetobacteraceae.Conclusion Acetobacter,Bacillus,Staphylococcus,Brevundimonas vesicularis,mold,Yeast and other microorganisms were isolated from the bactericidal lactobacillus beverage,most of which belongs to Acetobacteraceae.
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