Objective To study the detection method of 11a-progesterone and 11a-progesterone acetate by gel permeation chromatography and liquid chromatography,and to provide technical support for market supervision and enterprise quality control.Methods Samples are extracted by ethyl acetate;use 1:1 ethyl acetate:hexane as eluent;purify by gel permeation chromatography(GPC)method;collect the eluent for 12～20 min;obtain the purified solution from eluent by rotary evaporation;wait for the determination of liquid chromatography.Use 45:55 acetonitrile:water as mobile phase;use C18(250×4.6 mm)chromatographic column for separation;use photo diode array detector for the determination of 11a-progesterone and 11a-progesterone acetate at 245 nm.Results The linear correlation coefficient of this method is above 0.995,the recovery rate is 90.0%～102.6%and 85.1～102.7%,the relative standard deviation(RSD)is 0.7%～2.5%and 0.1%～8.7%,the limit of detection is 0.3 mg/kg and 0.6 mg/kg,and the limit of quantification is 1.0 mg/kg and 2.0 mg/kg.Conclusion The method is simple,reliable and accurate,and can be used for the determination of 11a-progesterone acetate and 11a-progesterone acetate in cosmetics.
high performance liquid chromatography11a-progesterone11a-progesterone acetatecosmetics
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