超高效液相色谱-质谱法鉴别蛤蚧定喘丸中的龟鳖甲类成分
Identification of Testudinis Carapax et Plastrum and Trionycis Carapax Form Ge jie Ding chuan Pill base on UPLC-MS/MS
殷红军 1吴越 1华小懿 1丁晴 1张瑾1
作者信息
- 1. 无锡市药品安全检验检测中心,无锡 214000
- 折叠
摘要
目的 建立蛤蚧定喘丸中龟鳖类成分的检测方法.方法 借鉴动物类药材特征肽鉴别方法,采用胰蛋白酶对不同批次的蛤蚧定喘丸样品进行酶解,利用超高效液相色谱-质谱(UPLC-MS/MS)法,选择龟鳖类特征分子离子峰作为检测离子对,ESI+模式,进行多反应监测模式检测.结果 8 家生产企业共 51 批蛤蚧定喘丸样品中均检出鳖甲成分,未检出其他龟鳖源成分.结论 该方法专属性较好,可用于蛤蚧定喘丸中龟鳖类成分掺伪检测.
Abstract
Objective To develop an ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)to identification of the feeding status of turtle and turtle shell components in different batches of Gejie Dingchuan Pills.Methods This study used the identification method of characteristic peptides of Animal medicine for reference,Enzymatic hydrolysis of different batches of Ge jie Ding chuan Pill samples using trypsin,and Identification by using the ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The characteristic ion pairs of Testudinis Carapax et Plastrum and Trionycis Carapax were as detection ion pairs.The positive electrospray ionization and multiple reaction monitoring were adopted.Result:51 batches of Gejie Ding chuan Pills form 8 companies have all detected turtle shell components,but no other turtle source components have been detected.Conclusion This method is raliable for identification of Testudinis Carapax et Plastrum and Trionycis Carapax form Ge jie Ding chuan Pill.
关键词
蛤蚧定喘丸/鳖甲/龟甲/特征肽/超高效液相色谱-质谱Key words
Gejie Ding chuan Pill/Testudinis Carapax et Plastrum/Trionycis Carapax/characteristic ion/UPLC-MS/MS引用本文复制引用
基金项目
江苏省市场监督管理局科技项目(KJ2023044)
中国药品监管科学行动计划重点项目(第二批)(NMPAJGKX-2023-045)
出版年
2024
NETL
NSTL
万方数据