Objective To develop an ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)to identification of the feeding status of turtle and turtle shell components in different batches of Gejie Dingchuan Pills.Methods This study used the identification method of characteristic peptides of Animal medicine for reference,Enzymatic hydrolysis of different batches of Ge jie Ding chuan Pill samples using trypsin,and Identification by using the ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The characteristic ion pairs of Testudinis Carapax et Plastrum and Trionycis Carapax were as detection ion pairs.The positive electrospray ionization and multiple reaction monitoring were adopted.Result:51 batches of Gejie Ding chuan Pills form 8 companies have all detected turtle shell components,but no other turtle source components have been detected.Conclusion This method is raliable for identification of Testudinis Carapax et Plastrum and Trionycis Carapax form Ge jie Ding chuan Pill.
Gejie Ding chuan PillTestudinis Carapax et PlastrumTrionycis Carapaxcharacteristic ionUPLC-MS/MS
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