Objective A method based on performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)has been developed for the determination of salbutamol in aquatic products and their products.Methods The analyte was enzymatic hydrolyzed in NH4AC solution overnight at 37℃,and extracted by ethyl acetate-isopropanol(1:1,v/v).The extract was cleaned up by QuEChERS method.Quantification was achieved by HPLC-MS/MS with multiple reaction monitoring(MRM)using internal standard method.Results The limit of quantification of salbutamol was 0.5 µg/kg.The average recoveries at three spiked levels of 0.5,1.0 and 5.0 µg/kg ranged from 87.8%to 110.7%with the relative standard deviations of 2.7%～9.6%(n=6).Conclusion The method has the advantages of simple pretreatment steps and its sensitivity,precision,and recoveries all meet the requirements of residue detection regulations,accurately solving the problem of lack of relevant technical support for monitoring salbutamol residues in aquatic products and their products.
aquatic products and their productssalbutamolhigh performance liquid chromatography-tandem mass spectrometry
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