Objective:This study aims to explore a method for determining the concentration of free amino acids in cell culture media based on ultra performance liquid chromatography(UPLC).Methods Researchers used UPLC technology combined with optimized sample processing Methods to efficiently and accurately determine free amino acids in cell culture medium.Results The research results indicate that the UPLC method can quickly and reliably determine the concentration of free amino acids in cell culture medium,providing an effective means for monitoring and optimizing cell culture in the biopharmaceutical industry.Conclusion Accurate determination of amino acid concentration not only helps to better understand the dynamic changes of key nutrients in cell culture media,but also provides important data support for process optimization in the production process.
ultra high performance liquid chromatographyamino acidscell culture mediummetabolism analysis
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