Objective To verify whether the laboratory can accurately detect fluoroquinolone residues in aquaculture products according to standard Methods.Methods In this article,according to the Methods of Determination of 17 Sulfonamides and 15 Quinolones Residues in Aquatic Products by Liquid Chromatography-Tandem Mass Spectrometry(Ministry of Agriculture Announcement No.1077-1-2008)and Laboratory Quality Control Specifications for Food Physical and Chemical Testing(GB/T 27404-2008),the method validation of the determination of 5 fluoroquinolones residues in cultured aquatic products by Liquid Chromatography-Tandem Mass Spectrometry was carried out,including linearity range,method sensitivity,accuracy and precision.Results The results showed that the 5 target compounds had good linear relationships in the range of 0～100 μg/kg,with correlation coefficients above 0.99.The method sensitivity(detection limit,quantification limit)met the method requirements,the average recovery rate was 93.0%～110%,and the relative standard deviation was≤15%,all of which met the method requirements.Conclusion It indicates that the laboratory can correctly apply this method for detection work under the existing detection conditions.
liquid chromatography-tandem mass spectrometryfarmed aquatic productsfluoroquinolonesmethod validation
NETL
NSTL
万方数据
