Objective To analyze and identify a strain numbered sznj2009.Methods A strain of sznj2009 was isolated from aquatic products,and the colony morphology,biochemical characteristics and biological characteristics of the strain were analyzed and identified.Results The strain showed round,smooth,small colorless colonies on the Vibrio chromogenic plate;Round,flat yellow colonies with opaque centers but transparent edges on CC and mCPC plates;Round,milky white colony on 3%sodium chloride tryptone soybean agar plate.By 16s rDNA sequence analysis found that the strain and vibrio harveyi(ATCC33843)closer,high homology,the evolution of the similarity of 100%.Conclusion The strain sznj2009 isolated from aquatic products was Vibrio harveyi.In the traditional biochemical identification and detection of Vibrio vulnificus,through comparison,it is found that Vibrio harveyi is similar to Vibrio vulnificus in colony characteristics and biochemical characteristics,however,the two strains can be clearly distinguished in the plate and biochemical reaction of Vibrio vulnificus,which can improve the identification accuracy of Vibrio vulnificus and provide reference for the traditional isolation and identification of Vibrio harveyi.
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