Objective To establish an UPLC method for monitoring the migration quantity of 14 antioxidants from brimonidine tartrate eye drop bottles.Methods The samples were separated by Waters ACQUITY UPLC BEH C18 chromatographic column with 0.02%formic acid solution-acetonitrile-tetrahydrofuran as mobile phase for gradient elution.The flow rate is 0.4 mL/min,the detection wavelength is 210 nm,the column temperature is 35℃,and the injection volume is 4 μL.Results The resolutions between the 14 antioxidants were greater than 1.5,and the 14 antioxidants have a good linear relationship in the concentration range from 0.2 μg/mL to 20 μg/mL(r＞0.9990).The specificity,precision,repeatability,accuracy,stability and durability of the method were all great.6 kind of antioxidants were detected in the 6 batches of samples from 6 companies,which were antioxidant-1076,168,2246,3114,445 and BHT.Conclusion The method is sensitive,rapid and easy to use with good repeatability,which is available for the determination of the migration quantity of 14 antioxidants from brimonidine tartrate eye drop bottles.
brimonidine tartrateeye dropsUPLCmigration of antioxidants
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