Objective To establish a method for the specific detection of adulteration of Panax notoginseng powder by fluorescence quantitative PCR.Methods After nucleic acid extraction of Panax notoginseng powder by an appropriate method,nucleic acid extract of authenticated authentic Panax notoginseng powder was used as the quantitative basis,and the specific primer was used for amplification by fluorescent quantitative PCR technique SYBR Green dye method.The standard curve was drawn with ΔRn as the ordinate and the pair value of percentage of content as the horizontal coordinate.A relative quantitative method of adulteration ratio of Panax notoginseng powder was established.Results In the range of relative content of 1%～20%,the correlation coefficient(r)was 0.998,and the linear relationship was good.The accuracy of the quantitative method was verified by artificially adulterated samples of 25%,50%and 75%,and the accuracy was between 103.2%and 122.6%,and the RSD was all less than 15%.Conclusion The linear range,accuracy and precision of the method were good,and it can be used for the quantitative determination of adulteration ratio of Panax notoginseng powder.In addition,the method can also be used to identify the authenticity of Panax notoginsengpowder because there was no specific expansion curve.
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