Objective By means of High Performance Liquid Chromatography(HPLC),the extraction method and content determination method of α-cyperone were explored to achieve the purpose of accuracy,reliability,convenience and easy operation.Methods High performance liquid chromatography was used.The chromatography was performed on Agilent Eclipse Plus(150×4.6 mm,5 μm)silica gel column with mobile phase consisted of methanol-water(70:30)and UV-VIS spectrometer.The flow rate was 1.0 mL/min.The detection wavelength was 240 nm.The column temperature was 35℃.Results The results showed a good linear relationship in the linear range of 0.02314-0.46287 mg/mL with R2=1,the average recovery rate of 99.84%and relative standard deviation(RSD)=0.09%.Conclusion The method has the advantages of simple operation,high sensitivity,good repeatability,accuracy and reliability,and can be used to determine the content of α-cyperone in order to control the quality of cyperone effectively.
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