The types and characteristics of hazards in university environmental laboratories are diverse,and the risk classification and prevention network barrier construction of hazards have a significant promoting effect on the management of hazards in university laboratories.The article adopts the method of system engineering to classify and statistically analyze the hazards in environmental laboratories;Based on the theory of accident risk chain,multi-level comprehensive evaluation method,and Murphy's law,a risk classification index system and prevention network barrier for environmental laboratory hazards have been constructed.The results indicate that:(1)The hazards in the laboratory are divided into two levels:risk factors and risk units.The risks of risk factors and risk units are divided into five levels:extreme danger,severe danger,significant danger,potential danger,and slightly dangerous.(2)Experimental materials,process equipment,environment,personnel,and safety management constitute the risk chain of experimental accidents.The occurrence of accidents is a chain event,and is greatly reduced by building layers of defense barriers.Building a prevention network barrier based on experimental projects,with division of labor and responsibility among all experimental personnel,interlocking of material,environmental,human and management,grading and precise supervision of hazardous units throughout the entire cycle,in all aspects,is a strong guarantee for laboratory safety.