首页|重组胶原蛋白液体敷料鉴别检验方法适用性研究

重组胶原蛋白液体敷料鉴别检验方法适用性研究

Applicability of identification and testing methods for recombinant collagen liquid dressings

扫码查看
重组胶原蛋白敷料是目前市场较火的一种皮肤护理类产品,可通过在创面形成保护层,起物理屏障作用.用于非慢性创面(如浅表性创面、激光/光子/果酸换肤/微整形术后创面)的护理,为创面愈合提供微环境.对敷贴类产品中所含的重组胶原蛋白鉴别的检测,是确保产品质量可靠的指标之一.本文研究了一款重组胶原蛋白液体敷料的鉴别方法的适用性.分别使用肽图法、N末端氨基酸序列法和等电聚焦电泳法对该试验样品中的重组胶原蛋白进行鉴别,检测结果发现肽图法、N末端氨基酸序列法对该样品具有适用性.而用现有的等电聚焦电泳法未发现有条带检出,该方法不适用.企业在选择重组胶原蛋白鉴别方法时应结合产品实际情况,对鉴别方法进行研究,采用适宜的方法.
Recombinant collagen dressings are currently a popular skin care product in the market,which can form a protective layer on the wound and act as a physical barrier.Used for the care of non chronic wounds(such as superficial wounds,laser/photon/fruit acid skin replacement/micro plastic surgery wounds),providing a microenvironment for wound healing.The detection of recombinant collagen protein in application products is one of the reliable indicators to ensure product quality.This article investigates the applicability of the identification method for a recombinant collagen liquid dressing.The applicability of a identification method for a recombinant collagen liquid dressing was studied.Peptide map method,N-terminal amino acid sequence method,and isoelectric focusing electrophoresis method were used to identify the recombinant collagen in the experimental sample,and the detection results showed that the peptide map method and N-terminal amino acid sequence method were applicable to the sample.However,the isoelectric focusing electrophoresis method did not detect any bands,so this method is not applicable.When choosing the identification method for recombinant collagen,enterprises should consider the actual situation of the product and adopt appropriate methods.

recombinant collagen proteinliquid dressingsmedical devicescollagen identification

陈永霞、张莉露、王朝壹、陈扬、张文杰

展开 >

天津市医疗器械审评查验中心,天津 3000191

纳通医用防护器材(天津)有限公司,天津 300308

重组胶原蛋白 液体敷料 医疗器械 胶原蛋白鉴别

2024

实验室检验
中国检验检测学会

实验室检验

ISSN:2097-261X
年,卷(期):2024.2(5)
  • 8