Fluorescence probe technology refers to a technical means of labeling the substance to be measured with fluorescence probe,enabling qualitative and quantitative analysis of the measured substance.Fluoride ion is one of the important trace elements in human body,proper intake of fluoride ion can help prevent tooth decay and osteoporosis,while excessive intake of fluoride ion can cause a variety of diseases.Therefore,it is of great significance to develop fluorescence probes that can detect fluoride ions in organisms.The latest scientific research results were converted into comprehensive designing experiment"the synthesis of fluorescence probe and detection of fluorine ions in aqueous phase".Through the implementation of the experiment,the students'ability of organic synthesis,the operation of optical analytical instruments and the analysis of experimental data will be greatly improved.