Objective A method was developed for the simultaneous determination of calcium,boron and sodium in Bombyx batryticatus by Inductively coupled plasma mass spectrometry(ICP-MS).Methods Microwave Digestion was used as the pretreatment method.The contents of Ca,B and Na in 25 batches of Bombyx batryticatus were determined by ICP-MS and analyzed by multivariate statistics.Results The three elements had a good linear relationship(r>0.995),the recoveries were in the range of 80%~120%,the reproducibility RSD<5%,the reproducibility RSD<5%and the stability RSD<5%within 1 day.Conclusion the method is simple and accurate,and can be used for the quantitative study of three elements in Bombyx batryticatus.The results can provide reference for the quality control and origin study of Bombyx batryticatus.