液相色谱法检测速溶奶茶中咖啡因含量
Determination of caffeine content in instant milk tea by liquid chromatography
魏艳艳 1周亮 1任波1
作者信息
- 1. 重庆开州区疾病预防控制中心,重庆 405499
- 折叠
摘要
目的 建立一种新的奶茶中咖啡因含量的液相色谱法,检测本区速溶奶茶咖啡因含量,提出健康影响建议.方法 采用waters高效液相色谱Symmetry C18(5 μm×150 mm×4.6 mm)柱进行测定,以甲醇水(30+70)为流动相,流速为1 mL/min,检测波长272 nm,柱温为35℃.结果 1～30 μg/mL线性范围内,相关系数r=0.9999,回收率为 97.2%～99.5%,方法精密度 0.54%.结论 该方法采取亚铁氰化钾和乙酸锌沉淀离心,能够迅速提取奶茶中咖啡因有效成分,并能去除蛋白质的干扰,缩短了前处理时间,优化了色谱条件,加速了咖啡因的检测.
Abstract
Objective To establish a new liquid chromatographic method for caffeine content in milk tea,to detect the caffeine content of instant milk tea in the region,and to make recommendations on health effects.Methods The determination was carried out on a Waters high performance liquid chromatography Symmetry C18 column(5 μm×150 mm×4.6 mm),the mobile phase was methanol-water(30+70)at a flow rate of 1 mL/min,the detection wavelength of 272 nm,and the column temperature was 35℃.Results The correlation coefficient was r=0.9999 over the linear range of 1-30 μg/mL,the recoveries were 97.2%-99.5%,and the method precision was 0.54%.Conclusion The method adopts potassiun ferrocyanide and zinc acetate precipitation centrifugation,which can rapidly extract the effective components of caffeine in milk tea and remove the interference of proteins,shorten the pretreatment time,optimize the chromatographic conditions,and accelerate the detection of caffeine.
关键词
高效液相色谱/奶茶/咖啡因Key words
high performance liquid chromatography/milk tea/caffeine引用本文复制引用
出版年
2024
NETL
NSTL
万方数据