Objective To establish a new liquid chromatographic method for caffeine content in milk tea,to detect the caffeine content of instant milk tea in the region,and to make recommendations on health effects.Methods The determination was carried out on a Waters high performance liquid chromatography Symmetry C18 column(5 μm×150 mm×4.6 mm),the mobile phase was methanol-water(30+70)at a flow rate of 1 mL/min,the detection wavelength of 272 nm,and the column temperature was 35℃.Results The correlation coefficient was r=0.9999 over the linear range of 1-30 μg/mL,the recoveries were 97.2%-99.5%,and the method precision was 0.54%.Conclusion The method adopts potassiun ferrocyanide and zinc acetate precipitation centrifugation,which can rapidly extract the effective components of caffeine in milk tea and remove the interference of proteins,shorten the pretreatment time,optimize the chromatographic conditions,and accelerate the detection of caffeine.
high performance liquid chromatographymilk teacaffeine
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