Objective To establish a method for benzo[α]pyrene determination in prepared Radix Rehmanniae by molecularly imprinted solid phase extraction coupled with ratio fluorescence detection.Methods The molecularly imprinted polymers(BaP-MMIPs)were prepared by sol-gel method using dendritic-grafting magnetic nanoparticle as the supporting material and benzo[α]pyrene as the template.Benzo[α]pyrene in the prepared sample was separated and enriched by BaP-MMIPs as dispersive SPE materials,and was detected by ratio fluorescence determination.The calibration curve was obtained by measuring the fluorescence intensity ratios(I436/I604)of benzo[α]pyrene in different concentrations.Results BaP-MMIPs showed remarkable selectivity,high efficiency and stable,which were successful applied as solid phase extraction materials for the trace benzo[α]pyrene determination in prepared Radix Rehmanniae.Good linearity was achieved in range of 0.14～3.0 μg/mL.The LOD was 80 ng/mL(S/N=3)and LOQ was 112.5 ng/mL(S/N=10).The average recoveries of benzo[α]pyrene were 89.5%-106.8%with the RSD≤3.6%.Conclusion The method is accurate,selective,high sensitive,reproducible,economical,simple and fast.The research has profound significance for the benzo[α]pyrene monitoring in Traditional Chinese Medicine.
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