New requirements have been put forward for biosafety in the new era,and it is also listed as a top priority.Medical universities are the cradle of medical talents.Microbiology,as an important subject in basic medicine which combine the theory and practice.Based on the biosafety problems existing in microbiology teaching laboratories,it is extremely urgent to optimization.Through analyzing the current situation of microbiology teaching laboratory,this article mainly discusses the current situation and optimization of microbiology teaching laboratories.In line with student-centered teaching conception,contains the safety awareness of teachers and students,laboratory safety management,and existing safety risks,so as to improve the scientific and specification of microbiology teaching laboratories.