Objective Clarify and optimize the detection methods for 9 antioxidants in oil samples.Methods After dissolving the oil sample in 3 mL of n-hexane solution saturated with acetonitrile,repeat the extraction 5 times with 3 mL of n-hexane saturated acetonitrile containing AP,and merge the 5 extraction solutions.Purify with a C18 solid-phase extraction column,collect and concentrate all extraction and eluent together to 0.2 mL,and finally make up to 2 mL with acetonitrile.Results 9 antioxidants linear relationship is good within the range of 1.0 to 60.0 μg/mL,and the correlation coefficients are all greater than 0.99;the recovery rate ranges from 91.2% to 108.8%,with a relative standard deviation(RSD)of 0.6% to 10.0%;the detection limit is within the range of 0.2～2.0 mg/kg,and the quantitative limit is within the range of 0.6～6.0 mg/kg.Conclusion This method optimizes the extraction frequency,purification method,and elution volume,and significantly improves the recovery rates of 9 antioxidants.The extraction frequency has a significant impact on the recovery rate of BHT and DG.
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