Objective To evaluate and analyze the value of halophilic bacteria culture in the detection of Vibrio parahaemolyticus in seafood in Dalian area.Methods 46 samples of Marine products were collected from Dalian from July 2023 to October 2023,and halophilic bacteria were cultured and isolated.The salt-tolerance test was carried out to identify and analyze the virulence of Vibrio parahaemolyticus,and the characteristics and detection of Vibrio parahaemolyticus in Marine products were analyzed.Results A total of 45 halophilic and salt tolerant strains were screened,with a yield of 44.44% at 20% salinity,55.56% at 15% salinity,and 11.11% at 50℃cultivation environment.The isolated strains continued to grow at a concentration of 25% NaCl,and the best growth was observed at a concentration of 10% NaCl.Seventeen moderately halophilic bacteria were detected,with a total of 28 salt tolerant strains.Conclusion The detection rate of Vibrio parahaemolyticus in Marine products in Dalian area can be improved by using halophilic bacteria culture detection.The results of virulence identification show that Vibrio parahaemolyticus has strong virulence and potential risk of food poisoning.Relevant departments must strengthen the detection of Vibrio parahaemolyticus in Marine products to ensure dietary safety.
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