荧光定量逆转录聚合酶链式反应法在病原微生物检验中的应用研究
Analysis of application results of fluorescence quantitative reverse transcription-polymerase chain reaction in pathogenic microorganism detection
郭金芳1
作者信息
- 1. 滨州市第二人民医院检验科,滨州 256800
- 折叠
摘要
目的 对荧光定量逆转录聚合酶链式反应(RT-PCR)法在病原微生物检验中的应用结果进行分析.方法 回顾性分析我院检验科于 2023 年 1 月 14 日—2023 年 12 月 14 日期间检验的 7502 份微生物检验资料,分别实施了常规RT-PCR法及荧光定量RT-PCR法检验,金标准为特殊染色镜检结果,分析两种检验结果的诊断效能等指标差异.结果 ①金标准检测出阴性、阳性占比为 490∶7012,常规RT-PCR法检验结果为阴性、阳性为:479∶6358,荧光定量RT-PCR法检验阴性、阳性比为:488∶7010.②荧光定量RT-PCR法的灵敏度为 99.97%、特异度为 99.59%、准确度为 99.95%,均高于常规RT-PCR法,有统计学意义(P＜0.05).③荧光定量RT-PCR法检验各类微生物检出率依次为99.91%、100.00%、100.00%、99.91%、100.00%、100.00%、99.97%,高于常规RT-PCR法,有统计学意义(P＜0.05).结论 荧光定量RT-PCR法在病原微生物检验中诊断效能及各类微生物检出率高,能够为医生诊疗提供科学依据,可推行.
Abstract
Objective To analyze the application results of fluorescence quantitative reverse transcription-polymerase chain reaction(RT-PCR)in the detection of pathogenic microorganisms.Methods A retrospective analysis was made of 7502 microbiological test data examined in the laboratory of our hospital from January 14th,2023 to December 14th,2023.Conventional RT-PCR method and fluorescent quantitative RT-PCR method were performed respectively,and the gold standard was the results of special staining microscopy.The diagnostic efficiency and other indicators of the two test results were analyzed.Results ① The ratio of negative to positive by gold standard was 490∶7012,the ratio of negative to positive by conventional RT-PCR was 479∶6358,and the ratio of negative to positive by fluorescence quantitative RT-PCR was 488∶7010.② The sensitivity,specificity and accuracy of fluorescence quantitative RT-PCR method were 99.97%,99.59%and 99.95%,which were higher than that of conventional RT-PCR method,with statisticalsignificance(P<0.05).③ The detection rates of various microorganisms by fluorescence quantitative RT-PCR were 99.91%,100.00%,100.00%,99.91%,100.00%,100.00%,99.97%,higher than that by conventional RT-PCR,with statistical significance(P<0.05).Conclusion Fluorescence quantitative RT-PCR method has high diagnostic efficiency and detection rate in pathogenic microorganisms,which can provide scientific basis for doctors'diagnosis and treatment and can be implemented.
关键词
荧光定量逆转录聚合酶链式反应法/病原微生物/检验/常规逆转录聚合酶链式反应法/支原体Key words
fluorescence quantitative reverse transcription-polymerase chain reaction method/pathogenic microorganism/inspect/conventional reverse transcription-polymerase chain reaction method/mycoplasma引用本文复制引用
出版年
2024
NETL
NSTL
万方数据