高效液相色谱-串联质谱法同时测定心通口服液中9种关键成分的含量
High-performance liquid chromatography-tandem mass spectrometry for simultaneous determination of nine components in Xintong Oral Liquid
耿征 1胡丹东1
作者信息
- 1. 北京市延庆区市场监管检验检测监控中心,北京 102100
- 折叠
摘要
目的 探讨高效液相色谱-串联质谱法同时测定心通口服液中 9 个成分的含量.方法 采用Phenomenex Luna-C18 色谱柱(250 mm×4.6 mm,5 μm),乙腈-甲醇-0.02 mol/L乙酸铵为流动相,梯度洗脱,体积流量0.9 mL/min.质谱条件:ESI源,MRM模式,定量离子对峰面积进行定量.结果 阿魏酸、二苯乙烯苷、葛根素等9种成分的线性范围为15.22～15220 ng/mL至25.36～25360 ng/mL.加样回收率分别为98.15%、101.84%,RSD为0.67%～1.64%.9批样品中,9种指标成分质量浓度范围为0.180～4.699 mg/mL.结论 本研究创新运用高效液相色谱-串联质谱法技术,高效精准地测定心通口服液九大核心成分,简化了流程,加速了质量控制,助力标准化生产.
Abstract
Objective To explore the simultaneous determination of the contents of nine components in Xintong Oral Liquid using high-performance liquid chromatography-tandem mass spectrometry.Methods A Phenomenex Luna-C18 column(250 mm×4.6 mm,5 μm)was used with acetonitrile-methanol-0.02mol/L ammonium acetate as the mobile phase for gradient elution at a flow rate of 0.9 mL/min.Mass spectrometry conditions:ESI source,MRM mode,quantification was performed using the peak area of the quantitative ion pairs.Results The linear ranges of the nine components,including ferulic acid,diphenylethylene glycoside,puerarin,etc.,were from 15.22 to 15220 ng/mL to 25.36 to 25360 ng/mL.The recovery rates were from 98.15%to 101.84%,with RSDs ranging from 0.67%to 1.64%.In nine batches of samples,the mass concentration ranges of the nine index components were from 0.180 to 4.699 mg/mL.Conclusion This study innovatively applies the HPLC-MS/MS technique to efficiently and accurately determine the nine core components in Xintong Oral Liquid,simplifying the process,accelerating quality control,and aiding in standardized production.
关键词
高效液相色谱-串联质谱法/心通口服液/成分含量Key words
high-performance liquid chromatography-tandem mass spectrometry/Xintong Oral Liquid/component content引用本文复制引用
出版年
2024
NETL
NSTL
万方数据