Objective To explore the simultaneous determination of the contents of nine components in Xintong Oral Liquid using high-performance liquid chromatography-tandem mass spectrometry.Methods A Phenomenex Luna-C18 column(250 mm×4.6 mm,5 μm)was used with acetonitrile-methanol-0.02mol/L ammonium acetate as the mobile phase for gradient elution at a flow rate of 0.9 mL/min.Mass spectrometry conditions:ESI source,MRM mode,quantification was performed using the peak area of the quantitative ion pairs.Results The linear ranges of the nine components,including ferulic acid,diphenylethylene glycoside,puerarin,etc.,were from 15.22 to 15220 ng/mL to 25.36 to 25360 ng/mL.The recovery rates were from 98.15%to 101.84%,with RSDs ranging from 0.67%to 1.64%.In nine batches of samples,the mass concentration ranges of the nine index components were from 0.180 to 4.699 mg/mL.Conclusion This study innovatively applies the HPLC-MS/MS technique to efficiently and accurately determine the nine core components in Xintong Oral Liquid,simplifying the process,accelerating quality control,and aiding in standardized production.
high-performance liquid chromatography-tandem mass spectrometryXintong Oral Liquidcomponent content
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