冷消解测定血清碘方法与国标法的比较分析
Comparative analysis of serum iodine determination by cold digestion method and national standard method
陈贵莲 1丁黎 1张颖 1李春春 2王晓2
作者信息
- 1. 美年大健康产业(集团)有限公司长沙公司,长沙 410000
- 2. 长沙塞克陆德医疗科技有限公司,长沙 410000
- 折叠
摘要
目的 评价冷消解测定血清碘方法在临床应用中的优势和价值.方法 应用长沙塞克陆德的DAT-500 型碘元素综合检测仪和冷消解血清碘检测试剂(专利:CN113340888A用于血清定量检测的试剂、试剂盒及检测方法)与国标《血清中碘的测定砷铈催化分光光度法》方法学适用性实验对比.结果 0～300 µg/L碘含量区间,5 批次标准曲线相关系数绝对值超 0.9995,检出限 2.1 µg/L;9 份不同浓度血清样加标回收率 94.6%～108.8%;3 种浓度血清样各测 6 批次,RSD为 3.41%～4.01%,样品加标回收率 94.6%～108.8%;20 例血清样本测定值与国标法差异无统计学意义(t=-1.660,P=0.113＞0.05).结论 冷消解法检测血清碘精度与准度高,操作简便耗时短、温控要求低,试剂常温易存运,应用前景佳.
Abstract
Objective To evaluate the advantage clinical value of cold digestion method and national standard method in the determination of serum iodine.Methods The DAT-500 iodine detector of Changsha Seclude and the cold digested serum iodine detection reagent(patent:CN113340888A reagent kit and detection method for serum quantitative detection)were used to compare with the national standard Determination of Iodine in Serum by Catalytic Spectrophotometry.Results From 0 to 300 μg/L iodine content range,the absolute value of correlation coefficient of standard curve of 5 batches exceeded 0.9995,the detection limit was 2.1 μg/L;the recovery rate of 9 serum samples with different concentrations was 94.6%～108.8%;6 batches of 3 serum samples with different concentrations were tested,the RSD was 3.41%～4.01%,and the recovery rate was 94.6%～108.8%;there was no statistical significance between 20 serum samples and national standard method(t=-1.660,P=0.113>0.05).Conclusion Cold digestion method has the advantages of high accuracy and accuracy,simple operation,short time consuming,low demand for temperature control,easy storage and transportation at room temperature.
关键词
冷消解血清碘检测试剂/血清碘自动检测仪/血清碘检测Key words
cold digestion serum iodine detection reagent/serum iodine automatic detection instrument/serum iodine detection引用本文复制引用
出版年
2024
NETL
NSTL
万方数据