首页|An integrative profiling of metabolome and transcriptome in the plasma and skeletal muscle following an exercise intervention in diet-induced obese mice

An integrative profiling of metabolome and transcriptome in the plasma and skeletal muscle following an exercise intervention in diet-induced obese mice

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Exercise intervention at the early stage of type 2 diabetes mellitus(T2DM)can aid in the maintenance of blood glucose homeostasis and prevent the development of macrovascular and microvascular complications.However,the exercise-regulated pathways that prevent the development of T2DM remain largely unclear.In this study,two forms of exercise intervention,treadmill training and voluntary wheel running,were conducted for high-fat diet(HFD)-induced obese mice.We observed that both forms of exercise intervention alleviated HFD-induced insulin resistance and glucose intolerance.Skeletal muscle is recognized as the primary site for postprandial glucose uptake and for responsive alteration beyond exercise training.Metabolomic profiling of the plasma and skeletal muscle in Chow,HFD,and HFD-exercise groups revealed robust alterations in metabolic pathways by exercise intervention in both cases.Overlapping analysis identified nine metabolites,including beta-alanine,leucine,valine,and tryptophan,which were reversed by exercise treatment in both the plasma and skeletal muscle.Transcriptomic analysis of gene expression profiles in the skeletal muscle revealed several key pathways involved in the beneficial effects of exercise on metabolic homeostasis.In addition,integrative transcriptomic and metabolomic analyses uncovered strong correlations between the concentrations of bioactive metabolites and the expression levels of genes involved in energy metabolism,insulin sensitivity,and immune response in the skeletal muscle.This work established two models of exercise intervention in obese mice and provided mechanistic insights into the beneficial effects of exercise intervention on systemic energy homeostasis.

type 2 diabetesexercise interventionmetabolomicstranscriptomics

Shuang Han、Qingqian Wu、Mengying Wang、Miqi Yang、Chen Sun、Jiaqi Liang、Xiaozhen Guo、Zheyu Zhang、Jingya Xu、Xinyuan Qiu、Cen Xie、Siyu Chen、Yue Gao、Zhuo-Xian Meng

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Department of Pathology and Pathophysiology and Department of Cardiology of the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China

Department of Geriatrics,Affiliated Hangzhou First People's Hospital,Zhejiang University School of Medicine,Hangzhou 310006,China

Key Laboratory of Disease Proteomics of Zhejiang Province,Zhejiang University School of Medicine,Hangzhou 310058,China

Department of Big Data in Health Science School of Public Health,Zhejiang University School of Medicine,Hangzhou 310003,China

State Key Laboratory of Natural Medicines and School of Life Science and Technology,China Pharmaceutical University,Nanjing 211198,China

State Key Laboratory of Drug Research,Shanghai Institute of Material Medical,Chinese Academy of Sciences,Shanghai 201203,China

Department of Biology and Chemistry,College of Liberal Arts and Sciences,National University of Defense Technology,Changsha 410073,China

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National Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaTraining Program of the Major Research Plan of the National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Fund for Excellent Young Scholars of ChinaZhejiang Provincial Natural Science Foundation of ChinaZhejiang Provincial Natural Science Foundation of ChinaInnovative Institute of Basic Medical Sciences of Zhejiang University,the Fundamental Research Funds for the Central UniversConstruction Fund of Medical Key Disciplines of HangzhouHangzhou Science and Technology BureauHangzhou Science and Technology BureauConstruction Fund of Key Medical Disciplines of HangzhouK.C.Wong Education Foundation

2018YFA08004032021YFC27019039185711081670740821009043200081781722012LZ21H070001LQ21C11000120150733Q13ZD20200129OO20200055

2023

10.1093/jmcb/mjad016

分子细胞生物学报(英文版)
中国科学院上海生命科学研究院,生物化学与细胞生物学研究所,中国细胞生物学学会

分子细胞生物学报(英文版)

CSTPCDCSCD
影响因子：0.595
ISSN：1673-520X
年,卷(期)：2023.15(3)
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