首页|SV2B defines a subpopulation of synaptic vesicles

SV2B defines a subpopulation of synaptic vesicles

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Synaptic vesicles can undergo several modes of exocytosis,endocytosis,and trafficking within individual synapses,and their fates may be linked to different vesicular protein composition.Here,we mapped the intrasynaptic distribution of the synaptic vesicle proteins SV2B and SV2A in glutamatergic synapses of the hippocampus using three-dimensional electron microscopy.SV2B is almost completely absent from docked vesicles and a distinct cluster of vesicles found near the active zone.In contrast,SV2A was found in all domains of the synapse and was slightly enriched near the active zone.SV2B and SV2A were found on the membrane in the peri-active zone,suggesting recycling from both clusters of vesicles.SV2B knockout mice displayed an increased seizure induction threshold only in a model employing high-frequency stimulation.Our data show that glutamatergic synapses generate molecularly distinct populations of synaptic vesicles and are able to maintain them at steep spatial gradients.The almost complete absence of SV2B from vesicles at the active zone of wildtype mice may explain why SV2A has been found to be more important for vesicle release.

3D electron microscopysynaptic vesiclessynaptic vesicle proteinsendocytosistraffickingspatial organization

Isabelle Paulussen、Hannes Beckert、Timothy F.Musial、Lena J.Gschossmann、Julia Wolf、Mathieu Schmitt、Jér?me Clasadonte、Georges Mairet-Coello、Christian Wolff、Susanne Schoch、Dirk Dietrich

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Synaptic Neuroscience Team,Department of Neurosurgery,University Hospital Bonn,Bonn 53127,Germany

Synaptic Neuroscience Team,Department of Neuropathology,University Hospital Bonn,Bonn 53127,Germany

Microscopy Core Facility,Medical Faculty,University of Bonn,Bonn 53127,Germany

UCB Pharma,Braine l'Alleud 1420,Belgium

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Deutsche Forschungsgemeinschaft(DFG)Deutsche Forschungsgemeinschaft(DFG)Deutsche Forschungsgemeinschaft(DFG)Deutsche Forschungsgemeinschaft(DFG)Deutsche Forschungsgemeinschaft(DFG)Deutsche Forschungsgemeinschaft(DFG)Deutsche Forschungsgemeinschaft(DFG)Deutsche Forschungsgemeinschaft(DFG)Deutsche Forschungsgemeinschaft(DFG)BONFOR program of the University of Bonn Medical Center(S.S.,D.D.)UCB Pharma

S.S.:SFB1089SCHO 820/4-1SCHO 820/6-1SCHO 820/7-1SCHO 820/5-2SPP1757D.D.:SFB1089SPP1757INST11

2023

10.1093/jmcb/mjad054

分子细胞生物学报(英文版)
中国科学院上海生命科学研究院,生物化学与细胞生物学研究所,中国细胞生物学学会

分子细胞生物学报(英文版)

CSTPCDCSCD
影响因子：0.595
ISSN：1673-520X
年,卷(期)：2023.(9)
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