首页|Sgo1 interacts with CENP-A to guide accurate chromosome segregation in mitosis

Sgo1 interacts with CENP-A to guide accurate chromosome segregation in mitosis

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Shugoshin-1(Sgo1)is necessary for maintaining sister centromere cohesion and ensuring accurate chromosome segregation during mitosis.It has been reported that the localization of Sgo1 at the centromere is dependent on Bub1-mediated phosphorylation of histone H2A at T120.However,it remains uncertain whether other centromeric proteins play a role in regulating the localization and function of Sgo1 during mitosis.Here,we show that CENP-A interacts with Sgo1 and determines the localization of Sgo1 to the centromere during mitosis.Further biochemical characterization revealed that lysine and arginine residues in the C-terminal domain of Sgo1 are critical for binding CENP-A.Interestingly,the replacement of these basic amino acids with acidic amino acids perturbed the localization of Sgo1 and Aurora B to the centromere,resulting in aberrant chromosome segregation and premature chromatid separation.Taken together,these findings reveal a previously unrecognized but direct link between Sgo1 and CENP-A in centromere plasticity control and illustrate how the Sgo1-CENP-A interaction guides accurate cell division.

mitosiscentromerekinetochoreSgo1CENP-AAurora B

Fengge Wu、Hameed Akbar、Chunyue Wang、Xiao Yuan、Zhen Dou、McKay Mullen、Liwen Niu、Liang Zhang、Jianye Zang、Zhikai Wang、Xuebiao Yao、Xiaoyu Song、Xing Liu

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MOE Key Laboratory for Cellular Dynamics,Center for Advanced Interdisciplinary Science and Biomedicine of Institute of Health and Medicine(IHM),University of Science and Technology of China School of Life Sciences,Hefei 230026,China

Anhui Key Laboratory for Cellular Dynamics and Chemical Biology,Hefei National Center for Cross-disciplinary Sciences,Hefei 230027,China

Keck Center for Cellular Dynamics,Morehouse School of Medicine,Atlanta,GA 30310,USA

Ministry of Science and Technology of ChinaMinistry of Science and Technology of ChinaMinistry of Science and Technology of ChinaMinistry of Science and Technology of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaStrategic Priority Research Program of the Chinese Academy of SciencesStrategic Priority Research Program of the Chinese Academy of SciencesMinistry of EducationMinistry of EducationMinistry of Education

2022YFA13031002022YFA08068002022YFA13027002017YFA0503600320900409225430292153302321707333162100222177106XDB19040000XDB37010105IRT_17R10220113402130010YD2070006001

2023

10.1093/jmcb/mjad061

分子细胞生物学报(英文版)
中国科学院上海生命科学研究院,生物化学与细胞生物学研究所,中国细胞生物学学会

分子细胞生物学报(英文版)

CSTPCDCSCD
影响因子：0.595
ISSN：1673-520X
年,卷(期)：2023.(10)
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