首页|原子力显微镜对牙釉质脱矿再矿化的分析

原子力显微镜对牙釉质脱矿再矿化的分析

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原子力显微镜是一种无须样本前处理的集形貌直观化和数据量化为一体的高分辨检测工具.利用原子力显微镜直观观察牙釉质脱矿及再矿化的微观形貌,并量化分析粗糙度和细菌黏附力.采用扫描电子显微镜和显微硬度计进行形貌观察和硬度值量化,验证原子力显微镜对牙釉质脱矿及再矿化的成像和量化效果.结果表明,随着脱矿时间的增加,牙釉质表面结构疏松,粗糙度增大;经过再矿化处理后,随着再矿化时间的增加,表面变得更为紧致,粗糙度降低.其他表征方法获得相似的结果,验证了原子力显微技术检测分析的可靠性.
Study on Demineralization and Remineralization of Tooth Enamel by Atomic Force Microscope
Atomic force microscopy(AFM)is a high-resolution inspection tool that integrates topography visualization and data quantification without sample pretreatment.In this study,AFM was used to visually observe the micromorphology of enamel demineralization and remineralization,and to quantify the roughness and bacterial adhesion force.Also,scanning electron microscopy and microhardness were used to quantify the morphology and hardness values of enamel demineralization and remineralization,which further verified the imaging and quantification effects of AFM.The results show that the surface structure is loose and the roughness increases with the increase of demineralization time.After remineralization,the surface becomes more compact and the roughness decreases with the increase of remineralization time.The other two characterizations obtained similar results,which verified the reliability of AFM detection.

atomic force microscopy(AFM)tooth enameldemineralizationremineralization

郝丽英、刘宇峰、文莺惠、郑赛男

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四川大学华西口腔医学院口腔疾病防治全国重点实验室,成都 610041

成都大学基础医学院,成都 610106

原子力显微镜 牙釉质 脱矿 再矿化

四川大学实验技术项目

SCU1076

2024

实验室研究与探索
上海交通大学

实验室研究与探索

CSTPCD北大核心
影响因子:1.69
ISSN:1006-7167
年,卷(期):2024.43(1)
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