The Practice and Discussion of Fluorescence Staining Technology in Cell Biology Experiments
Pollen tube is a model system for studying plant cell morphogenesis and polar growth,and its rapid growth relies on efficient transport of membrane vesicles.Using lily pollen tubes,this study seeks to improve experimental teaching courses on the plasma membrane and endomembrane system,and establishes an experimental project for observing vesicle trafficking and pollen tube growth with fluorescence staining technology.This project covers multiple knowledge points,such as germination of lily pollen,observation of cytoplasmic flow,and fluorescence staining and observation of the transport of mitochondria and membrane vesicles.The design,implementation,results and discussion of the experiments greatly advance students'understanding of membrane components and the endomembrane system,and improve the quality and effectiveness of experimental teaching.The present exploration and practice of experimental teaching reform fully take advantage of fluorescence staining technology,a key method commonly used in experimental courses and research,and expand the content of experimental courses in cell biology.This exploration exercises students'scientific research thinking,improves their scientific literacy and innovation ability,and promotes the cultivation of outstanding innovative talents in basic disciplines.
cell biology experimentexperiment teachingfluorescence stainingpollen germinationvesicular transport
万方数据
维普
