首页|虚拟仿真在"生物技术药物学实验"中的应用

虚拟仿真在"生物技术药物学实验"中的应用

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由于许多实验环境无法满足新冠病毒取样和检测的要求,且病原微生物新冠病毒具有强传染性和致病性,学生很难开展感染性实验操作.因此,将虚拟仿真技术与新冠病毒实时荧光定量PCR检测相结合构建了虚拟实验平台,其中涵盖实验导学、临床取样及实时荧光定量PCR检测等主要内容,重点模拟RNA提取、病毒序列获取与分析、引物设计、反转录、实时荧光定量PCR及结果分析等操作.通过虚拟环境,在保障学生安全的基础上,让学生更深入地了解新冠病毒实时荧光定量PCR的检测,大大提高了生物技术药物学实验教学的效率.
Application of Virtual Simulation in"Biotechnical Drug Experiments"
Since the experimental environment of the majority of schools cannot meet the requirements of sampling and detection of neocoronavirus in performing real-time fluorescence quantitative PCR,and neocoronavirus is highly infectious and pathogenic during operation,it is difficult for students to carry out the actual experimental operation.Therefore,the virtual simulation technology combined with the real-time fluorescence quantitative PCR detection of the new coronavirus is used to build a virtual experimental platform,which covers the main content of the experimental introduction,clinical sampling and real-time fluorescence quantitative PCR detection,focuses on the simulation of the RNA extraction,viral sequence acquisition and analysis,primer design,reverse transcription,the development of standard curves,real-time fluorescence quantitative PCR and analysis of the results of the operation.Through the virtual environment,students can have a deeper understanding of the detection of real-time fluorescence quantitative PCR of new coronavirus,which greatly improves the efficiency of the experimental teaching of biotechnology pharmacology.

virtual simulation technologybiotechnical drug experimentsreal-time fluorescence quantitative PCR detection of COVID-19

张倩倩、徐立朋、马志国、郭钟雪、陆小云、王峰

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暨南大学药学院,广州 510632

虚拟仿真技术 生物技术药物学实验 新冠病毒的实时荧光定量PCR检测

2023年度第一批暨南大学"金课"建设项目(港澳台侨特色"金课"专项)广州市应用基础研究项目广东省自然科学基金面上项目广东省自然科学基金面上项目2024年度暨南大学教学质量与教学改革工程项目

101000132022010100102022A15150126362024A1515013257JD2024003

2024

10.19927/j.cnki.syyt.2024.08.031

实验室研究与探索
上海交通大学

实验室研究与探索

CSTPCD北大核心
影响因子：1.69
ISSN：1006-7167
年,卷(期)：2024.43(8)
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