首页|RP-HPLC法同时测定叶下珠中没食子酸、柯里拉京和短叶苏木酚的含量

RP-HPLC法同时测定叶下珠中没食子酸、柯里拉京和短叶苏木酚的含量

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目的 建立反相高教液相色谱法同时测定叶下珠中没食子酸、柯里拉京和短叶苏木酚的含量.方法 采用Kromasil C18色谱柱(250mm × 4.6mm,5μm),流动相为乙腈-体积分数0.1％磷酸水溶液梯度洗脱,流速1.0 mL·min-1,检测波长为280 nm,柱温30℃.结果 没食子酸、柯里拉京和短叶苏木酚的质量浓度分别在5.300～106.7 mg·L-1(r=0.999 8)、2.00～40.6 mg·L-1(r=0.9997)、0.270 ～5.42 mg·L-1(r =0.999 7)内与峰面积呈良好的线性关系.没食子酸、柯里拉京和短叶苏木酚的平均加样回收率(n=9)分别为97.9％、101.7％、100.2％,RSD分别为1.8％、1.8％、2.7％.结论 该分析方法准确可靠,重复性好,为更好地控制叶下珠药材的质量提供方法.
Simultaneous determination of gallic acid, corilagin and brevifolin in Phyllanthus urinaria by RP-HPLC
Objective To establish an HPLC method for simultaneous determination of gallic acid,corilagin and brevifolin in Phyllanthus urinaria. Methods The chromatographic separation was achieved on a Kromasil C18 (250 mm × 4. 6 mm, 5 μm) column with acetonitrile-0. 1 % phosphoric acid solution as mobile phase ( gradient elution). The flow rate was 1. 0 mL·min-1, the column temperature was maintained at 30 ℃, and the detective wavelength was set at 280 nm. Results The calibration curves of gallic acid,corilagin and brevifolin were linear in the range of 5.300-106.7 mg·L-1 (r = 0.999 8), 2.00 -40.6 mg·L-1 (r=0.999 7), 0.270-5.42 mg·L-1 (r = 0.999 7),respectively. The average recoveries(n =9) were 97.9%, 101.7%, 100. 2% ;and the RSDs were 1. 8%, 1.8%, 2.7% .respectively. Conclusions The method is reliable, accurate and reproducible for quality control of P. urinaria.

Phyllanthus urinariaRP-HPLCgallic acidcorilaginbrevifolincontent determination

闫小玉、孙蒙、杨国光、毕开顺、陈晓辉

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沈阳药科大学药学院,辽宁沈阳110016

沈阳药科大学中药学院,辽宁沈阳110016

叶下珠 RP-HPLC 没食子酸 柯里拉京 短叶苏木酚 含量测定

2012

沈阳药科大学学报
沈阳药科大学

沈阳药科大学学报

CSTPCDCSCD北大核心
影响因子：0.604
ISSN：1006-2858
年,卷(期)：2012.29(1)
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