Barrier repair therapy of the fucoidan in Sargassum fusiforme via a MAPK-dependent mechanism in epidermal
Objective To investigate the effect of fucoidan from Sargassum fusiforme fucoidan(SFF)on the repair of epidermal barrier injury,and to evaluate the effects of SFF on the proliferation,migration and differentiation of keratinocytes.Methods In the present study,the effects of SFF on epidermal permeability barrier recovery were examined.Besides,the effects of SFF on proliferation,migration and differentiation in keratinocytes were evaluated,which was critical to properly form the epidermal barrier.Moreover,the effects of SFF on epidermal permeability barrier recovery and normal skin were also evaluated.Results As revealed from our data,SFF could noticeably promote the proliferation of HaCaT cells under a dose-dependent effect.The treatment with SFF at a lower concentration(2.5 μg·mL-1)also effectively promoted HaCaT cells to migrate.Moreover,SFF up-regulated the gene expression of differentiation markers in HaCaT cells,and promoted the proliferation,migration and differentiation of epidermal keratinocyte by activating ERK and JNK pathways.Furthermore,the recovery rate and hydration of epidermal permeability barrier after tape stripping were significantly increased in the SFF-treated group as compared with the vehicle-treated group(P＜0.05).Conclusion SFF displays comprehensive functions to regulate the disordered keratinocytes and can be used as a potential ingredient in functional cosmetics and therapeutic agents to treat cutaneous disorders that are associated with abnormal epidermal barrier function.
万方数据
维普
