The effects of different infection methods on rabies virus proliferation in human diploid cells
Objective To investigate the effect of infection of human diploid cells by virus-carrying passage,direct adsorption and mixed passage on the proliferation of rabies virus.Methods After infection with different methods,the rabies virus harvesting solution was obtained from the manufacturing in the cell plant,and the vaccine stock solution was prepared by filtration and clarification,concentration,purification and inactivation.The virus titer of the harvesting solution and antigen content of the stock solution were determined using the assay method of Chinese Pharmacopoeia(2020).Results The virus titers of the harvesting solutions for virus-carrying passage infection group,mixed passage infection group and direct adsorption infection group were(6.40±0.33),(6.35±0.18)and(4.71±0.39)lgLD50·mL-1,respectively.The antigen contents of the stock solutions were(34.8±1.5),(34.3±2.1)and(12.6± 2.0)IU·mL-1,respectively.Conclusion After the rabies virus PV strain infected human diploid cells MRC-5 cells by mixed passage,good virus proliferation was shown with a clear viral strain generation and a simple process.Therefore,this method was more suitable for the manufacturing of rabies vaccine.
万方数据
维普
