Objective An analytical method based on solid phase extraction combined with high performance liquid chromatography was established for the determination of Sennoside A and Sennoside Bin weight-reducing tea.Methods After supersonic extractionwith 70％(volume fraction)methanolas solvate,the extracts were purified by anion-exchange solid phase column chromatography,eluted with methanol containing 2％(volume fraction)ammonia,and concentrated in a stream of nitrogen to obtain the final sample solutions.These samples were separated with a BDS HYPERSIL C18 chromatographic column,using methanol and 0.1％phosphoric acid(volume fraction)as the mobile phase,analyzed using a diode array detector and quantified with reference to an external standard.Results Sennoside A and SennosideBhad good linearity in the mass concentration range of 1-100 μg·mL-1,quantitative limits were 10 mg·100 g-1.The average recoveries were in the range from 92.2％to 98.4％(n=6),relative standard deviations were in the range of0.8％-3.3％(n=6).Conclusion This method accordingly proved to be suitable for the determination of Sennoside A and Sennoside Bin weight-reducing tea.
solid phase extractionHPLCweight-reducing teaSennoside ASennoside B
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