Carbon dots-polydopamine nanocomposite for synergistic tumor therapy
Objective To improve drug efficacy and reduce toxicity,a specific drug delivery system based on carbon dots(CDs)modified polydopamine(PDA)was constructed.Methods CDs with a diameter of ap-proximately 4 nm were prepared by hydrothermal method.Then,CDs were modified on the surface of PDA to prepare PDA@CDs carriers.Afterwards,doxorubicin hydrochloride(DOX)was used as the model drug to prepare DOX/PDA@CDs.Transmission electron microscopy(TEM),Zeta potential,dynamic light scat-tering(DLS)and fluorescence spectroscopy were used to characterize the drug loading system.In vitro re-lease behaviors of the drug loading system were investigated by simulating the tumor microenvironment and applying NIR light irradiation.Cell level experiments were conducted to investigate the synergistic effect of thermotherapy and chemotherapy.Results PDA@CDs had excellent photothermal property and photothermal stability.The in vitro release profile showed that without NIR light irradiation and pH 7.4 PBS,the release a-mount of DOX was low.However,under high concentration of H2O2,pH 5.0 PBS,and NIR light irradia-tion,the release of DOX increased significantly.Cellular uptake experiments proved that PDA@CDs could be uptaken by cells and emit green fluorescence.Cytotoxicity tests demonstrated that DOX/PDA@CDs showed brilliant chemo/photothermal synergistic effect on killing tumor cells after NIR irradiation.Conclu-sion DOX/PDA@CDs has pH/H2O2/NIR multi-responsive drug release characteristics,which can realize chemo/photothermal synergistic effect,thus owning a bright prospect in the field of cancer treatment.
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维普
