Preparation and optimization of β-carotene nanoemulsion and its formulation technology
Objective To investigate the advantages and disadvantages of β-carotene nanoemulsion modified by polysialic acid,and to explore the optimal formulation of β-carotene nanoemulsion by the central point design response surface method.Methods Firstly,the solubility of β-carotene was used as an indicator to screen the optimal oil phase in this study;Then,the centrifugal stability constant,entrapment efficiency and drug loading were regarded as indicators,and the optimal prescription and preparation process were selected by using the central point design response surface method.The particle size and Zeta potential were measured by Zetasizer Nano ZS90,the release profiles in vitro and thermal stability of nanoemulsion were explored,and the physicochemical properties of nanoemulsion were characterized with these indexes.Results The optimal formulation of β-carotene nanoemulsion modified by polysialic acid was consist of β-carotene of 0.4％(w),medium chain triglyceride(MCT)of 25％(w),poly sialic acid-octadecylamine derivative(PSA-C18)of 4％(w)and Span 80 of 0.4％(w).The nanoemulsion exhibited small and uniform particle size,relative high surface Zeta potential and significant slow release behavior in vitro.It was preliminarily demonstrated that nanoemulsion modified by polysialic acid could efficiently load β-carotene and improve thermal stability of β-carotene.Conclusion By optimizing the formulation and technology of β-carotene nanoemulsion modified by polysialic acid,it will provide theoretical basis and technical support for the development and application of β-carotene nanoemulsion.
β-carotenepolysialic acidnanoemulsioncentral point design response surface method
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