Research progress and application of cytochrome P450-RhFRED artificial fusion enzyme
P450RhF from Rhodococcus sp.NCIMB 9784 is a self-sufficient cytochrome P450 enzyme,whose heme domain is mainly responsible for controlling the substrate specificity of the enzyme,the redox partner RhFRED naturally fuses with the P450 heme domain.Electrons were transferred within the protein molecule.Based on the self-sufficiency of P450RhF,some scholars successively constructed artificial fusion enzyme P450-RhFRED with other P450s by using the redox partner RhFRED of P450RhF to construct functional P450 enzymes.The P450-RhFRED fusion protein library is of great significance for the exploration of the catalytic function of unknown P450s.P450-RhFRED artificial fusion proteins can also be used in development of enzymes for industrial applications.
万方数据
维普
