Isolation and identification of chemical constituents from Hypericum perforatumn L.
Objective To study the chemical composition of Hypericum perforatum L.Methods Various chromatographic methods such as normal-phase silica gel chromatography,reversed-phase silica gel chromatography,high performance liquid chromatography and gel chromatography were used to separate and purify the 95%EtOH extract of this plant.Structures of the isolated compounds were identified by NMR,UV,and IR spectral,high resolution mass spectrum(HRM),and X-ray single crystal diffraction.Results Eight compounds were isolated and identified from this plant,namely:perforxanthone A(1),8-hydroxy-1,2,3-trimethoxyxanthone(2),aspidinol D(3),(S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(4),ent-4(15)-eudesmene-1β,6α-diol(5),methyl-(E)-8-oxooctadec-9-enoate(6),moronic acid(7),betulinic acid(8).Conclusion Compound1 is a new compound.Compounds2-7 are isolated from this plant for the first time.
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