DNA barcoding analysis of introduced Dendrobium officinale from different cultivation bases
Objective To explore the molecular genetic differences of Dendrobium officinale Kimura et Migo cultivated in different provinces,and to provide reference for germplasm selection,cultivation and breeding.Methods In this study,30 samples of cultivated D.officinale from five provinces were collected,and their intraspecies genetic analysis was investigated by ITS and chloroplast barcodes.Results The results showed that some cpDNA barcodes could detect variations of D.officinale from different habitats,while the intraspecific variation in ITS was inadequate:trnH-psbA is 790 bp(1 indel,2 SNP),pbsI-pbsK is 473 bp(3 indels,1 SNP),trnF-ndhJ is 682 bp(1 SNP)and ITS is 551 bp(2 SNP).In cpDNA barcodes,the samples of D.officinale from Guizhou had their specific haplotypes,and the samples from Anlong,Guizhou was the closest to D.nobile.ITS analysis showed that the D.officinale samples of Zhejiang was close to D.nobile in the evolutionary tree.Conclusion In general,D.officinale from Guizhou has higher genetic diversity,suggesting that it might origin from multiple germplasm sources.The genetic distance between D.officinale samples from Guizhou and Zhejiang is closer to D.nobile,indicating that they may belong to an earlier branch in the evolutionary process,and other samples from other provinces are likely to be derived from it.This study shows that although it is not possible to distinguish the origin of D.officinale in different regions by intraspecific genetic variation,DNA barcodes can be used to reveal the genetic differences of D.officinale from different cultivation regions and provide a certain scientific basis for the cultivation and breeding of D.officinale.
万方数据
维普
