Profiling of the chemical composition of phenanthrenes in Bletilla striata based on UPLC-Q-TOF/MS technology
Objective To rapidly identify chemical compounds of phenanthrenes in Bletilla striata by UPLC-Q-TOF/MS and integrated qualitative strategy.Methods The UPLC conditions are as follows:ACQUITY UPLC HSS T3 reverse phase column(100 mm×2.1 mm,1.8 μm),column temperature of 40℃,mobile phase of acetonitrile(A)-0.1%formic acid aqueous solution(B),and flow rate of 0.4 mL·min-1;Xevo G2 Q-TOF mass spectrometry and electrospray ionization(ESI)source were used to collect data in negative ion modes.Integrated qualitative strategies were adopted,including mass defect filter,product ion filter,neutral loss filter and chemical structure retention relationship analysis,to screen all chemical components in Bletilla striata.Through the establishment of compound database,automatic screening of UNIFI platform,comparison of reference substances and manual confirmation,a total of 104 phenanthrenes compounds were characterized in Bletilla striata.Results A total of 104 chemical compounds of phenanthrenes in Bletilla striata were characterized.Conclusion The established method can quickly and accurately characterize the chemical compounds of phenanthrenes in the Bletilla striata,which provides scientific basis for the study of pharmacological substance basis and quality control of Bletilla striata.
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