Co-loaded gambogic acid and Cypate nanoparticles for synergetic chemotherapy and photothermal therapy of cancer
Objective The poly(lactic-co-glycolic)(PLGA)nanoparticles(GA-Cy-PNs)loaded with gambogic acid(GA)and the photothermal agent Cypate was prepared,to achieve the combination of chemotherapy and photothermal therapy and enhance antitumor effect.Methods GA-Cy-PNs were fabricated through an oil-in-water(O/W)emulsion technique.The morphological features of the nanoparticles were observed through a transmission electron microscopy.The average size,polydispersity index,Zeta potential and the stability of nanoparticles were determined by dynamic light scattering.The drug loading efficiencies and the coating rates of GA and Cypate were quantified by a UV-visible spectrometer.The GA-Cy-PNs releasein vitro was studied by dialysis method.The photothermal effect and photothermal stability of GA-Cy-PNs were recorded using an infrared thermal imaging camera.The cellular uptake behaviors of GA-Cy-PNs in 4T1 and HepG2 cells with or without the NIR laser irradiation were studied using confocal laser scanning microscopy.The cytotoxicity of the nanoparticles on 4T1 and HepG2 cells was investigated by a CCK-8 assay.Results The nanoparticles exhibited a uniform spherical shape with high dispersity and good stability.The encapsulation efficiencies of GA and Cypate were measured to be 80.76%and 82.73%,and the drug loading efficiencies were 3.51%and 7.19%,respectively.Under acidic condition,GA can be rapidly released from the nanoparticle.The photothermal test demonstrated that GA-Cy-PNs had good photothermal conversion properties and excellent photothermal stability.The results of the in vitro biosafety showed that GA-Cy-PNs had the good safety and compatibility.NIR laser irradiation could promote the nanoparticles uptake of 4T1 and HepG2 cells.The cytotoxicity test confirmed that the combination of chemotherapy drugs and photothermal therapy can achieve stronger cytotoxicity than that of photothermal therapy alone or chemotherapy alone.Conclusion GA-Cy-PNs can achieve chemo-photothermal combination therapy and possess an obvious antitumor efficacy,providing a new research idea for the synergistic treatment of cancer.
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