Objective The chemical constituent′s investigation of Ohwia caudata(Thunberg)H.Ohashi leaves was carried out to enrich its material basis.Methods The dried leaves of O.caudata were extracted by heating reflux to obtain 70%EtOH extraction.Then,the extraction was separated and purified by various chromatographic methods to gain compounds,whose structures were identified by using NMR and mass spectra mainly.Results Fifteen C-glycosyl flavonoids named as isovitexin(1),vitexin(2),6,8-di-C-β-D-glucopyranosylapigenin(3),isosaponarin(4),isovitexin-2″-O-rhamnoside(5),swertisin(6),isoswertisin(7),2″-O-rhamnosylswertisin(8),2″-α-rhamnopyranosyl-7-O-methylvitexin(9),luteolin 6-C-β-glucopyranoside(10),luteolin 8-C-β-glucopyranoside(11),isoorientin-2″-O-rhamnoside(12),swertiajaponin(13),2″-O-rhamnopyranosyl-7-O-methylisoorientin(14),and isoscoparin-7-O-glucoside(15)were obtained from the 70%ethanol extract of O.caudata leaves.Conclusion Among them,compounds 4,5,7,11-15 are gained from Ohwia genus for the first time.
the leaves of Ohwia caudata(Thunberg)H.Ohashi.C-glycosyl flavonoidsstructural identificationrestricted rotational isomerism