Inhibitory effects of acid responsive baicalin liposomes on tumor cell proliferation and immune sensitization
Objective An acid-responsive baicalin-immunoliposome(baicalin-liposome-1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly(2-ethyl-2-oxazoline,BA-LP-PEOz,BA-LP-PEOz)was synthesized to enhance the antitumor therapeutic efficacy of baicalin.By leveraging tumor-specific drug delivery mechanisms and chemosensitization processes,this formulation aims to achieve high therapeutic effect with minimal adverse effects.Methods Baicalin-loaded immunoliposomes were synthesized using the film dispersion method,and the physicochemical characteristics of the liposomes were assessed.In vitro evaluations of cell growth inhibition were conducted using lung cancer A549 cells,while cell growth proliferation assay was performed using RAW264.7 macrophages.Results The encapsulation rate of BA-LP-PEOz was determined to be(84.3±0.3)%,with a particle size of(135±5)nm and a Zeta potential of(-14.9±1.4)mV.BA-LP-PEOz exhibited potent direct cytotoxic effects on A549 cells at the optimal administration concentration(BA mass concentration of 100 μg·mL-1).and showed the ability to promote proliferation in RAW264.7 macrophages and enhance macrophage polarization towards the M1 phenotype.Conclusion BA-LP-PEOz can improve the anti-tumor therapeutic efficiency of BA by playing the dual roles of chemotherapy and immunotherapy.
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