Research progress of catalytic hairpin self-assembly(CHA)optimization
Nucleic acid has long been considered more than just a repository of genetic information,but enjoys a broad prospect in the fields of nanotechnology and bioanalytical applications.catalytic hairpin self-assembly(CHA),with its high sensitivity,constant temperature amplification,enzyme-free condition,simple operation and the ability to combine various technologies and detect a wide range of substances,has been highly favored ever since its inception.In recent years,much research has been conducted to improve the performance of the CHA circuit by studying the special design of the hairpin structure,combining other nucleic acid amplification techniques or start-up methods,etc.However,due to the overabundance of the methods for performance optimization and the difficulty of categorization,there are few related literature reviews in this area.This paper will compile the literature related to CHA circuit optimization in terms of both its initiation and conduction,introduce and analyze the CHA circuit,and offer some views on the prospects and challenges of CHA circuit optimization.It is hoped that this paper may contribute to the building of the CHA circuit with better performance in the future.
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