The effect of triptolide on the proliferation,invasion and apoptosis of osteosarcoma cell SaOS-2 through lncRNA BE503655
Objective To study the effect of triptolide on the proliferation,invasion and apoptosis of osteosarcoma cell SaOS-2.Methods SaOS-2 osteosarcoma cells were divided into Control group,Triptolide group(40,80,160 nmol·L-1 triptolide),positive control group(15 mg·L-1 5-fluorouracil),Triptolide+si-NC group(transfected siRNA control,160 nmol·L-1 triptolide),Triptolide+si-BE503655 group(transfected with BE503655 siRNA,160 nmol·L-1 triptolide),CCK-8 experiment was used to analyze cell changes,flow cytometry was used to analyze cell apoptosis,and Transwell chamber was used to analyze cell migration and invasion changes,Western blot was used to analyze N-cadherin,E-cadherin,Bax,Bcl-2,MMP-2 protein expression changes,qRT-PCR method was used to analyze the expression of BE503655.Results Compared with the control group,the survival rate of osteosarcoma cells in the Triptolide group(40,80,160 nmol·L-1 triptolide)and positive control group were decreased,the apoptosis rate was increased,and the number of invasion and migration were decreased.Bax,E-cadherin protein expression level were increased,Bcl-2,MMP-2,N-cadherin protein expression level was decreased,and BE503655 expression level was increased.Compared with the Triptolide+si-NC group,the osteosarcoma cell survival rate in the Triptolide+si-BE503655 group was increased,the apoptosis rate was decreased,the number of migration and invasion were increased,and the protein expression levels of MMP-2,N-cadherin and Bcl-2 were increased,and the expression levels of E-cadherin and Bax protein were decreased,the BE503655 level was decreased.Conclusion Triptolide exerts the effects of inhibiting proliferation,migration,invasion,EMT and promoting apoptosis of osteosarcoma cell SaOS-2 by up-regulating BE503655.
万方数据
维普
