Expression of LncRNA ITGB1 in pituitary tumors and its influence on cell biological behavior
Objective To detect the expression of long non-coding RNA(LncRNA)ITGB1 in pituitary tumors and to explore its effects on the proliferation,apoptosis,invasion and migration behavior of pituitary tumor cells.Methods Forty-eight patients with pituitary tumors and 48 tissue donors who died of accidents who underwent surgical treatment in our hospital from September 2018 to April 2021 were selected to collect pituitary tumor tissue and normal anterior pituitary tissue respectively.Human pituitary tumor RC-4BC cells were divided into control group,siRNA NC group(transfected with nonsense siRNA),LncRNA ITGB1 siRNA group(transfected with LncRNA ITGB1 siRNA),pcDNA3.1 NC group(transfected with pcDNA3.1)and pcDNA3.1 LncRNA ITGB1 group(transfected with pcDNA3.1 LncRNA ITGB1),qPCR method was used to detect the expression of LncRNA ITGB1 in RC-4BC cells,Western Blot method was used to detect the protein expression of proliferating cell nuclear antigen(PCNA),cysteinyl aspartate specific proteinase-3(Caspase-3)and matrix metalloproteinase-9(MMP-9).Results The expression level of LncRNA ITGB1 in pituitary tumor tissue was higher than that in normal anterior pituitary tissue;The expression of LncRNA ITGB1 in pituitary tumor tissues of different tumor size and invasive patients was different statistically;compared with the siRNA NC group,the RC-4BC cell proliferation rate,invasion number,scratch healing rate,LncRNA ITGB1 level,PCNA and MMP-9 protein levels in the LncRNA ITGB1 siRNA group were reduced,the apoptotic rate of RC-4BC cells and the level of Caspase-3 protein in the cells were increased;compared with pcDNA3.1 NC group,the RC-4BC cell proliferation rate,invasion number,scratch healing rate,LncRNA ITGB1 level,PCNA and MMP-9 protein levels in the pcDNA3.1 LncRNA ITGB1 group were increased,the apoptotic rate of RC-4BC cells and the level of Caspase-3 protein in the cells were reduced.Conclusion LncRNA ITGB1 is up-regulated in pituitary tumor,and related to the tumor size and invasion in patients with pituitary tumor,it can promote the proliferation,invasion and migration of pituitary tumor RC-4BC cells,and inhibit cell apoptosis.
long non-coding RNA ITGB1pituitary tumorproliferationapoptosis
万方数据
维普
