Study on quality evaluation of Gardenia jasminoides processed by alum based on fingerprint of different polar parts and physicochemical properties
Objective HPLC fingerprints of different polar parts of Gardenia jasminoides were established,and the distribution of chemical components of Gardenia jasminoides was systematically studied.Based on this,the rationality and necessity of alum boiling treatment of Gardenia jasminoides were explored in combination with the experiments of color value and antioxidant activity,so as to provide reference for comprehensive evaluation of the quality of Gardenia jasminoides and rational clinical drug use.Methods The samples were extracted by water bath heating reflux method,and the HPLC fingerprints of different polar parts of Gardenia jasminoides were collected respectively.The color values and antioxidant activities were detected by different processing methods.Cluster analysis,principal component analysis and other stoichiometric methods were used for comprehensive comparative study.Results There were 11 common peaks for petroleum ether,9 for chloroform,9 for ethyl acetate and 17 for n-butanol.The color value of Gardenia jasminoides retreated with alum was significantly higher than that retreated with water,but there was no significant difference in antioxidant activity.All the stoichiometric analysis results showed that there was little difference in chemical components of the polar parts of Gardenia jasminoides retreated with alum and that retreated with water.Conclusion Alum treatment mainly plays a role in fixing and increasing the color of Gardenia jasminoides in industrial production,has no obvious effect on the medicinal quality of prepared slices of Gardenia jasminoides.The HPLC fingerprints of different polar parts established in this study were rich in chromatographic information,with good peak type and separation degree,which could comprehensively reflect the distribution of chemical components,and provide reference for the overall quality evaluation of Gardenia jasminoides.
万方数据
维普
