Effect of Sijunzi decoction on immunotoxicity induced by mitomycin C
Objective To explore the attenuation effect of Sijunzi Decoction(SJZD)on immunotoxicity induced by mitomycin C(MMC).Methods Mouse spleen lymphocytes were isolated and cultured in vitro.Conanin A(ConA)was used to stimulate cell proliferation.MMC,SJZD and the co-treatment were used to co-culture with spleen lymphocytes respectively.ELISA was used to detect the effects of different drugs on the levels of interleukin-2(IL-2)and interferon-γ(IFN-γ)in splenic lymphocyte culture medium supernatant.Results Compared with control group(CON),the cell viability of MMC group was significantly decreased after 24 and 48 h,and the decrease was more obvious at 48 h(*P<0.05,**P<0.01).After 24 h,SJZD group promoted cell growth in the range of 25-200 μg·mL-1 and cell viability was prominently increased at 100 μg·mL-1(*P<0.05),inhibited cell growth in the range of 400-800 μg·mL-1,and SJZD(25-800 μg·mL-1)combined with MMC group(COM)increased cell viability compared with MMC group.After 48 h,SJZD group promoted cell growth in the range of 25-200 μg·mL-1 and cell viability was significantly increased at 100-200 μg/mL(*P<0.05),inhibited cell growth in the range of 400-800 μg·mL-1.The cell viability of COM group(100-200 μg·mL-1)was obviously increased compared with that of MMC group(#P<0.05).Compared with CON group,the levels of IL-2 and IFN-γ in MMC group were prominently reduced,SJZD(100 and 200 μg·mL-1)groups were significantly increased(*P<0.05,**P<0.01).COM groups were significantly increased compared with MMC groups(#P<0.05,##P<0.01).Conclusion SJZD can promote the growth of immune cells and the secretion of cytokines(IL-2 and IFN-γ),reduce the inhibition of MMC to splenic lymphocytes and the secretion of IL-2 and IFN-γ to a certain extent.
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