Promotion of acute myeloid leukemia progression by lncRNA-XIST via suppression of miR-186-5p and up-regulation of PSME3 expression
Objective To investigate the mechanism of long intergenic noncoding RNA X-inactive specific transcript(lncRNA-XIST)promoted acute myeloid leukemia(AML)progression by suppressing miR-186-5p.Methods Kaplan-meier was used to analyze the correlation between XIST expression and survival in 32 AML patients.KG-1 cells were tranfected with miRNA mimic,miRNA inhibitor or PSME3 shRNAs.Subsequently,the mRNA expression levels of XIST and miR-186-5p were detected by quantitative real-time PCR.The protein expression levels of PSME3,cleaved-caspase 3 and cleaved-caspase 8 were detected by Western-blotting.Cell viability detected by CCK-8 assay and flow cytometry.Results XIST were significantly expressedby AML patients and AML cell lines.Knocking down XIST inhibited the proliferation and promoted the apoptosis of KG-1 cells.Moreover,when KG-1 cells with XIST knockdown were transplanted into nude mice,tumor formation and progression were remarkably suppressed.Dual-luciferase reporter assay showed that miR-186-5p targeted XIST.The mechanism investigation showd that XIST endogenously competed sponge miR-186-5p regulate PSME3 expression.Conclusion XIST endogenously competed as a sponge for miR-186-5p to regulate PSME3 expression in AML,and the aberrant expression of XIST is a potential molecular target for AML diagnosis and therapy.
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