Norcantharidin induces apoptosis through Wnt/β-catenin signaling pathway in human ovarian cancer SKOV3 cells
Objective:To investigate the effect of Norcantharidin(NCTD)on the apoptosis of human ovarian cancer SKOV3 cells and its mechanism.Methods:Human ovarian cancer SKOV3 cells were cultured in vitro and treated with 52 μmol/L NCTD.The SKOV3 cells were divided into the control group and NCTD group(52 μmol/L).The morphological changes of SKOV3 cells were observed under an inverted microscope,and the changes of nucleus were observed under a fluorescence microscope.The changes of mitochondrial membrane potential were detected by flow cytometry after drug treatment for 24 h.Western blot was used to detect the expression levels of apoptosis-related proteins Bax,Bcl-2 and Wnt/β-catenin signaling pathway-related protein β-catenin in SKOV3 cells after drug treatment for 24 h.Result:Compared with that in the control group,NCTD inhibited the proliferation of SKOV3 cells,reduced mitochondrial membrane potential,induced apoptosis of SKOV3 cells,increased the expression of Bax protein,decreased the expression of Bcl-2 protein and β-catenin.Conclusion:NCTD-induced apoptosis of ovarian cancer SKOV3 cells may be related to the regulation of Wnt/β-catenin signaling pathway.
norcantharidinapoptosisovarian cancer SKOV3 cellWnt/β-catenin signaling pathway
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